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Caliper Technologies Corp. (605 Fairchild Dr., Mountain View, CA 94043-2234; Tel: 650/623-0700, Fax: 650/623-0500) has developed microfluidic devices that allow in-line labeling of enzyme (protease inhibition) assays to be performed in a continuous-flow format.
According to Hua Yang, a Caliper researcher, a protease assay is performed using unlabeled (non-fluorogenic substrates), and then the products are labeled using fast amino derivatization chemistry. Due to the fast derivatization, the labeling step is performed as the products are produced and/or flowed through the system.
The method, therefore, provides a rapid, one step …