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A surveillance system used different detection methods to estimate prevalence of Shiga toxin-producing Escherichia coli during 2003-2005 and 2001-2002. More non-O157 serotypes were detected by enzyme immunoassay than by evaluation of non-sorbitol-fermenting E. coli isolates. We therefore recommend use of enzyme immunoassay and culture-based methods.
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Infection with Shiga toxin-producing Escherichia coli (STEC) is a frequent cause of gastrointestinal disease, particularly among children and elderly persons (1). Detection of O157 STEC by culture relies primarily on sorbitol MacConkey agar (SMAC) (2) because O157:H7 strains cannot rapidly ferment ...