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SYBR green-based assay detects 100% of West Nile virus target region variants.

Hematology Week

| June 28, 2004 | COPYRIGHT 2009 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

2004 JUN 28 - (NewsRx.com & NewsRx.net) -- A SYBR green-based assay is able to detect 100% of West Nile virus target region variants.

According to published research from the United States, "Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus (WNV). Rapidly evolving RNA viruses present a challenge for diagnosis because they accumulate mutations that may render them undetectable."

"To explore the effect of sequence variations on assay performance, we generated every possible single point mutation within the target region of the widely used TaqMan assay for WNV and found that the TaqMan…

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Source: HighBeam Research, SYBR green-based assay detects 100% of West Nile virus target region...

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