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Comparative effects of heat shock gene silencing in renal ischaemia.(Nephrology)
Introduction: Over expression of HSP72 or HSP25 augments recovery of cellular polarity in injured renal epithelial cells. To evaluate the relationship between heat shock response and recovery from ATP depletion, HSP72 and HSP25 were differentially inhibited using transcription factor decoy or short interference RNA (siRNA).
Method: Pre-transcriptional heat shock inhibition was achieved using double stranded, circular, heat shock transcription factor decoy oligonucleotides. Post transcriptional inhibition was provided by siRNA targeted at an inducible porcine HSP72 gene. Porcine proximal renal tubular cells were injured for 2 h by incubation with substrate deplete …
