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Tat-neutralizing antibodies compared in vaccinated macaques.(AIDS vaccine development)(Brief Article)
2003 APR 16 - (NewsRx.com & NewsRx.net) -- Researchers at the Institute of Human Virology, at the University of Maryland, compared antisera of macaques immunized with Tat or chemically modified Tat toxoid having the same clade B sequence "for their abilities to recognize diverse Tat sequences."
I. Tikhonov and colleagues explained that they constructed "an overlapping peptide array covering three clade B and two clade C Tat sequences ... to help identify reactive linear epitopes."
They reported, "Sera from Tat-immunized macaques were broadly cross-reactive with clade B and clade C sequences but recognized a clade B-specific epitope in the basic domain. Sera from Tat toxoid-immunized macaques had a more restricted pattern of recognition, reacting mainly with clade B and with only one clade B basic domain sequence, which included the rare amino acids RPPQ at positions 57 to 60.
"Monoclonal antibodies against the amino terminus or the domain RPPQ sequence blocked Tat uptake into T cells and neutralized Tat in a cell-based ...
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