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The new human cell line EN has a TP53 mutation and a 1-day doubling time.(uterine adneocarcinoma)
2003 APR 10 - (NewsRx.com & NewsRx.net) -- The new human cell line EN has a TP53 mutation and a 1-day doubling time.
According to a study from Japan, they created "a new cell line, EN, established from an invasive endometrioid adenocarcinoma of the uterine corpus in a 50 yr old patient. The cells show rapid growth in culture with a doubling time of 24.4 hrs and high migration activity."
"Monolayer cultured cells were polygonal in shape and showed a tendency to pile up without contact inhibition. Subcutaneous transplantation of the EN cells into nude mice formed solid tumors that were histologically diagnosed as adenocarcinoma, whereas no metastasis was observed. Cultured EN cells produced tissue polypeptide antigen. Genetic and molecular analyses revealed high telomerase activity and estrogen receptor beta, but not alpha, expression. Using PCR single strand conformation polymorphism technique, we have screened EN cells for TP53 mutation in exons 5-8. A mobility shift was observed in this cell line in exon 8. A nucleotide insertion (CGT-->CAGT) was detected at codon 273, which resulted in a creation of a stop codon at codon 308," stated K. Isaka and coauthors from Tokyo Medical University.
Isaka and coauthors concluded: "This cell ...
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