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Dendritic cells generated in serum-free medium using cytokine combinations.
2003 APR 9 - (NewsRx.com & NewsRx.net) -- Antigen-loaded dendritic cells were generated in a serum-free medium using different cytokine combinations.
According to a study from Czech Republic, "Dendritic cells (DCs) are antigen-presenting cells that play a critical role in the induction of cytotoxic T-lymphocytes. An optimal method for the generation of DC for clinical use remains to be established. The aim of our study was to find an optimal cytokine combination for DC generation from peripheral blood stem cells (PBSC) and peripheral blood mononuclear cells (PBMC) in serum-free conditions."
"Serial immunophenotyping enabled us to observe changes in DC content during the culture as well as the development of maturation and activation markers," reported Tomas Buchler and collaborators at Masaryk University Hospital. "As a source for DC culture, we used PBSC from patients with multiple myeloma after stem cell mobilization using cyclophosphamide and G-CSF, or PBMC from healthy donors without mobilization. The cells were cultured in a serum-free medium with different cytokine combinations including GM-CSF, TNF-alpha, Flt-3, CD40L, IFN-gamma, IL-1alpha, IL-6, PGE1, and IL-4. The cell cultures were evaluated by immunophenotyping. For PBMC, interleukin-12 assay was performed."
The investigators found, "For PBSC, the yield of DC as determined by CD83+ cell count ranged from 0.6 x 10[superscript]5 to 30.1 x 10[superscript]4 (mean: 9.4 x 10[superscript]4) of DC generated ...
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