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Vaccination with plasmids of infectious bursal disease virus protective.
2003 APR 2 - (NewsRx.com & NewsRx.net) -- DNA vaccination with plasmids containing various fragments of large segment genome of infectious bursal disease virus protected chickens against disease.
"The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (STC) strain were successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) followed by cloning into a eukaryotic expression plasmid vector (pCR3.1) as DNA vaccines," researchers in the United States report.
"Transient expression of the encoded genes from various constructed plasmids was characterized in COS-7 cells by positive immunofluorescent staining with polyclonal or monoclonal antibody to IBDV," said Hua-Chen Chang and colleagues at Purdue University. "Chickens (1-day old) were intramuscularly injected with the individual plasmid three times at weekly intervals, challenged with IBDV strain STC at 21-day old, and observed for 10 days. Chickens receiving the plasmids containing VP2 genes, including VP243, VP24, or VP2 fragment, did not have clinical signs, mortality, and bursal atrophy and were effectively protected against IBDV infection.
"On the contrary, chickens receiving plasmids ...
- Vaccination with plasmids of infectious bursal disease virus protective.
- Newspaper article from: Immunotherapy Weekly April 2, 2003 700+ words
...large segment genome of infectious bursal disease virus protected chickens...large segment genome of infectious bursal disease virus (IBDV) in the...protection of chickens against infectious bursal disease (IBD). Different fragments...
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- Newspaper article from: Immunotherapy Weekly June 2, 2004 700+ words
...partial protection against infectious bursal disease virus. "Several DNA...capsid protein gene of infectious bursal disease virus (IBDV) injected...Partial protection against infectious bursal disease virus through DNA-mediated...
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- Newspaper article from: Virus Weekly January 27, 2004 700+ words
...as variant strains of infectious bursal disease virus using reverse genetics...as variant strains of infectious bursal disease virus using reverse genetics...major subject areas of Infectious Bursal Disease Virus, Vaccine Development...
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...Bursamune(R) in ovo, an infectious bursal disease (IBD) vaccine, has...are protected against infectious bursal disease with post-hatch vaccines...for inoculating against infectious bursal disease (IBD). Embrex also...
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- Press release article from: PR Newswire May 4, 1999 700+ words
...that Bursamune(TM), an infectious bursal disease (IBD) vaccine, has received...challenge producers face from bursal disease. Among the top 10 producing...vaccine for inoculating against infectious bursal disease (IBD). Embrex also is...
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- Press release article from: PR Newswire December 4, 2002 700+ words
...approval for Bursaplex(R) infectious bursal disease (IBD) vaccine from the...response to this approval since bursal disease remains a threat to flocks...safely protect poultry from bursal disease when maternal antibodies...
Source: HighBeam Research, Vaccination with plasmids of infectious bursal disease virus...