Ovalbumin allergenic peptide effective for allergic airway inflammation.

2003 APR 2 - (NewsRx.com & NewsRx.net) -- An E. coli strain carrying an ovalbumin allergenic peptide as a fused protein to OMPC was therapeutic in a murine model of allergic airway inflammation.

According to a study from Mexico, "An Escherichia coli strain expressing the ovalbumin (OVA) 323-329 allergenic peptide on the bacterial surface was evaluated for its ability to reduce the lung inflammatory response in mice allergic to OVA."

"BALB/c mice were rendered allergic by means of two intraperitoneal injections of OVA suspended in alum 5 days apart, and one intratracheal boost 1 week later," reported Sara Huerta Yepez at Hospital de Infectologia "Dr. Daniel Mendez Hernandez" Centro Medico "La Raza" IMSS and collaborators throughout Mexico. "The mice were then treated with two intranasal, one week apart, doses of 4 x 10[superscript]9 E. coli-UH302 transformed with plasmids pST13 or pST13-OVA(323-339), which bear the OmpC porin from Salmonella enterica serovar Typhi or the OmpC with the OVA allergenic 323-339 amino acid sequence inserted in the external loop 5."

"The allergic inflammatory reaction was evaluated on day 31, finding that mice treated with E. coli-UH302-pST13-OVA reduced four to seven times perivascular and peribronchial infiltrates, mucus production, goblet cell hyperplasia, and eosinophils when compared with mice treated with E. coli-UH302-pST13 or saline solution," stated the investigators. "These results were ...