AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Promoter trap system increases expression of protective antigen.
2003 APR 2 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - Vaccines containing live attenuated Bacillus anthracis or Bacillus subtilis expressing recombinant protective antigen (rPA) were made more potent by adding a promoter trap system based on based on various gfp reporter genes, according to a report in Infection and Immunity.
"Accordingly, a B. anthracis library of 6000 clones harboring plasmids with chromosomal B. anthracis DNA fragments inserted upstream from gfpuv was constructed," reported Orit Gat and colleagues at the Israel Institute for Biological Research in Ness Ziona. "Based on fluorescence intensity, 57 clones carrying potentially strong promoters were identified, some of which were DNA sequenced."
The investigators found that Pntr (251 bp) was the most potent promoter for B. anthracis, increasing its expression of rPA 500-fold over the native pagA promoter and 70-fold over Pamy, the alpha-amylase promoter.
"The number of cell-associated pre-PA molecules in B. anthracis was found to correlate well with the strength of the promoter," said Gat and associates (Use of a promoter trap system in Bacillus anthracis and Bacillus subtilis for the development of recombinant protective antigen-based vaccines. Infect Immun, 2003;71(2):801-813).
The level of mature PA secreted appeared to have an upper boundary, however, and did not surpass that allowed by Pamy. In addition, when ...
- PharmAthene Response to DHHS Request for Proposals for Recombinant Protective...
- Press release article from: PR Newswire September 15, 2008 700+ words
...s proposal for its recombinant protective antigen anthrax vaccine, SparVax(TM...Proposals for an Anthrax Recombinant Protective Antigen (rPA) Vaccine for the Strategic...novel second generation recombinant protective antigen (rPA) anthrax vaccine being developed...
- Calcium may play role in folding pathway of anthrax protective antigen.
- Newspaper article from: Medical Letter on the CDC & FDA January 25, 2004 700+ words
...role in the folding pathway of the anthrax protective antigen. "Protective antigen (PA) is the central receptor binding...Conformational fluctuations in anthrax protective antigen: a possible role of calcium in the folding...
- PharmAthene Submits Response to DHHS Request for Proposals for SparVax(TM) - A...
- Press release article from: PR Newswire July 31, 2008 700+ words
...Proposals for an Anthrax Recombinant Protective Antigen (rPA) Vaccine for the Strategic...Proposals, for an Anthrax Recombinant Protective Antigen Vaccine for the Strategic National...a second generation recombinant protective antigen (rPA) anthrax vaccine, (2) Valortim...
- Specific, sensitive, and quantitative enzyme-linked immunosorbent assay for...
- Magazine article from: Emerging Infectious Diseases Quinn, Conrad P. Semenova, Vera A. Elie, Cheryl M. Romero-Steiner, Sandra Greene, Carolyn Li, Han Stamey, Karen Steward-Clark, Evelene Schmidt, Daniel S. Mothershed, Elizabeth Pruckler, Janet Schwartz, Stephanie Benson, Robert F. Helsel, Leta O. Holder, Patricia F. Johnson, Scott E. Kellum, Molly Messmer, Trudy Thacker, W. Lanier Besser, Lilah Plikaytis, Brian D. Taylor, Thomas H., Jr. Freeman, Alison E. Wallace, Kelly J. Dull, Peter Sejvar, Jim Bruce, Erica Moreno, Rosa Schuchat, Anne Lingappa, Jairam R. Marano, Nina Martin, Sandra K. Walls, John Bronsdon, Melinda Carlone, George M. Bajani-Ari, Mary Ashford, David A. Stephens, David S. Perkins, Bradley A. October 1, 2002 700+ words
...antibodies to Bacillus anthracis protective antigen (PA) in human serum. The qualified...quantitative ELISA for detection of anti-protective antigen (PA) specific immunoglobulin...Preparation Recombinant anthrax toxin protective antigen (rPA) with an amino acid sequence...
- Emergent BioSolutions Acquires Advanced Recombinant Protective Antigen Anthrax...
- Press release article from: Business Wire May 5, 2008 700+ words
...acquisition of all assets and rights related to a recombinant protective antigen (rPA) anthrax vaccine product candidate and related...candidate, rPA 102, is based on a recombinant form of the protective antigen protein. This vaccine contains a purified protein (rPA...
- PharmAthene Submits Response to DHHS Request for Proposals for SparVax(TM) - a...
- News wire article from: Bioterrorism Week August 11, 2008 700+ words
...to a Request for Proposals for an Anthrax Recombinant Protective Antigen (rPA) Vaccine for the Strategic National Stockpile...as a Request for Proposals, for an Anthrax Recombinant Protective Antigen Vaccine for the Strategic National Stockpile. The solicitation...
- Patent No. 07601351 Issued on Oct. 13, Assigned to Human Genome Sciences, Inc....
- News wire article from: US Fed News Service, Including US State News October 15, 2009 700+ words
...Laytonsville, US, have developed a Antibodies against protective antigen .The inventors were issued U.S. Patent No. 07601351...antibodies and related molecules that specifically bind to protective antigen of (PA). Such antibodies have uses, for example...
- Emergent BioSolutions Submits Proposal in Response to HHS RFP for Development...
- Press release article from: Business Wire July 31, 2008 700+ words
...of Health and Human Services (HHS) for a recombinant protective antigen anthrax vaccine (rPA). Emergent's rPA vaccine candidate...candidate, rPA 102, is based on a recombinant form of the protective antigen protein. This vaccine contains a purified protein (rPA...
Source: HighBeam Research, Promoter trap system increases expression of protective antigen.