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Freeze-thaw cycles do not hinder detection of measles, mumps, rubella antibodies.(Brief Article)

Vaccine Weekly

| March 12, 2003 | COPYRIGHT 2003 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

2003 MAR 12 - (NewsRx.com & NewsRx.net) -- A series of freeze-thaw cycles do not hinder the detection of measles, mumps, or rubella antibodies.

"We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays," researchers in the United States report.

"Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each," said Norman A. Pinsky and colleagues at the Mayo Clinic and Olmsted Medical Group in Rochester, Minnesota. "Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees Celsius until assayed. Each assay measurement was done in replicates of five, and the mean value was reported."

The investigators reported, "After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on ...

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