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Formulation parameters significantly affect vaccine microsphere performance.
2003 MAR 5 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - The formulation of alginate microspheres can affect the hydrophobicity, cellular uptake, and macrophage toxicity of the microspheres significantly, according to a report in the Journal of Controlled Release.
"Favorable interaction with macrophages is critical for uptake subsequent processing of the microspheres used for oral vaccine delivery," said Argaw Kidane and colleagues at Pharmacia Corporation, Amgen, and Purdue University.
The investigators prepared five different types of alginate microspheres through emulsion cross-linking methods: alginate alone (A; mean size of 11 micrometers), alginate with methylcellulose (AA; mean size of 10.5 micrometers), AA with Pluronic L61 (AA61; mean volume size of 3.8 micrometers), alginate with hydroxypropyl methylcellulose (AK3; mean size of 8.7 micrometers), and AK3 with Pluronic L61 (AK3 61; mean size of 3.9 micrometers. The volume sizes of the microspheres after coating with poly-L-lysine (PLL) were 10.4, 10, 3.7, 8.8, and 3.5 micrometers, respectively.
Hydrophobicity values, as determined by contact angle on a glass slide, for A, AA, AA61, AK3, AK361 were 20, 34.8, 71, 29, and 80 degrees, respectively. Hydrophobicity values increased to 49.7, 55.8, 91, 48.25, and 84.4 degrees, respectively, after the microspheres were coated with PLL.
"Cellular uptake studies using flow cytometry revealed that AA61 microspheres coated with PLL were phagocytosed most often by mouse macrophages," reported the researchers. "Toxicity to macrophages was shown to depend on the ratio of microspheres to cells."
Cellular uptake ...
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Source: HighBeam Research, Formulation parameters significantly affect vaccine microsphere...