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Nonculture-based techniques useful for studying vaginal microbiota.(Brief Article)
2003 MAR 6 - (NewsRx.com & NewsRx.net) -- According to recent research from Canada, "the vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE)
and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women were studied further to investigate the effect and persistence of vaginally inserted capsules containing viable lactobacilli."
"PCR-DGGE indicated that most subjects had a microbiota represented by one to three dominant DNA fragments," said J.P. Burton and colleagues, St. Josephs Hospital, Lawson Health Research Institute.
"Analysis of these fragments revealed that 79% of the women possessed sequences with high levels of similarity to Lactobacillus species sequences. Sequences homologous to Lactobacillus iners sequences were the most common and were detected in 42% of the women tested. Alteration of the vaginal microbiota could be detected by PCR-DGGE in several women after the instillation of lactobacilli," they said.
"Additionally, randomly amplified polymorphic DNA analysis of lactobacilli isolated from selective media demonstrated that the exogenous strains could be detected ...
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...diagnostic methods employed (Hogan et al. 2007). At any given time, an estimated 29% of women have an aberrant vaginal microbiota indicative of this condition, even if they are not all symptomatic (Allsworth and Peipert 2007). Recurrent...
Source: HighBeam Research, Nonculture-based techniques useful for studying vaginal...