Chromosome analysis detects cell lines ideal for studying gene transfer.

2003 MAR 6 - (NewsRx.com & NewsRx.net) -- Researchers in Australia have identified several ovarian cancer cell lines that would be ideal candidates for studying cancer gene therapy, based on their analysis of chromosome 8.

"The short arm of chromosome 8 undergoes frequent loss of heterozygosity (LOH) in ovarian adenocarcinomas. Fine mapping has identified several distinct critical regions within 8p which undergo rates of LOH of 50% or greater, suggesting that there may be more than one tumor suppressor gene located on this chromosome arm. In an effort to refine the location of these putative tumor suppressor genes by homozygosity-mapping-of-deletion analysis, we have analyzed 21 ovarian cancer cell lines with 19 polymorphic microsatellite markers from 8p," stated J.M. Arnold and coauthors, Queensland Institute for Medical Research, Bramston Terrace.

"Eleven of the cell lines (55%) were homozygous at every marker, indicating loss of an entire 8p arm. No smaller extended regions of hemizygosity were identified. Refinement of these 8p target regions was therefore not possible, but this analysis did identify the ovarian cancer cell lines that would be most appropriate for microcell-mediated chromosome transfer to complement the hypothesized mutation in the target tumor suppressor gene(s) on 8p," said researchers.

"The 11 cell lines that had undergone 8p LOH were therefore characterized for ...