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Novel promoter in human papillomavirus type 18 genome found.(Brief Article)
2003 FEB 5 - (NewsRx.com & NewsRx.net) -- "Northern blot and RT-PCR analyses indicated that the human papillomavirus E4 open reading frame is expressed in HeLa cells," researchers in the United States report.
"Because integration at the E1 or E2 open reading frame would place the viral upstream regulatory region downstream of the viral late genes, the expression of E4 in HeLa cells is most likely regulated by host cellular promoter(s) or unidentified viral promoter(s) in the E2 region," wrote Kelly Y. Kim and colleagues at Indiana University. "Primer extension analysis and transient transfection experiments with luciferase reporter constructs under the transcriptional control of various subgenomic fragments of HPV-18 were carried out to identify and characterize functional promoters within the E2 region."
The researchers demonstrated "the in vivo activity of a novel promoter located within the ...
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Source: HighBeam Research, Novel promoter in human papillomavirus type 18 genome found.(Brief...