AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Development of real-time fluorescence PCR assay to detect diphtheria toxin gene.(Brief Article)
2003 FEB 5 - (NewsRx.com & NewsRx.net) -- Researchers from the United States report the development and evaluation of "a real-time fluorescence PCR assay for detecting the A and B subunits of diphtheria toxin (tox) gene. When 23 toxigenic Corynebacterium diphtheriae strains, 9 nontoxigenic C. diphtheriae strains, and 44 strains representing the diversity of pathogens and normal respiratory flora were tested, this real-time PCR assay exhibited 100% sensitivity and specificity."
"It allowed for the detection of both subunits of the tox gene at 750 times greater sensitivity (2 CFU) than the standard PCR (1,500 CFU)," said Elizabeth A. Mothershed and collaborators at the Centers for Disease Control and Prevention in Atlanta. "When used directly on specimens collected from patients with clinical diphtheria, one or both subunits of the tox gene were detected in 34 of 36 specimens by using the real-time PCR assay; only 9 specimens were found to be positive by standard PCR. Reamplification by standard PCR and DNA sequencing of the amplification product confirmed all real-time PCR tox-positive reactions."
The ...
- Development of real-time fluorescence PCR assay to detect diphtheria toxin gene.
- Newspaper article from: Medical Letter on the CDC & FDA February 9, 2003 700+ words
...evaluation of "a real-time fluorescence PCR assay for detecting the A and B subunits...specimens by using the real-time PCR assay; only 9 specimens were found...Development of a real-time fluorescence PCR assay for rapid detection of the...
- LightCycler PCR assay detects/characterizes enterotoxigenic Escherichia coli.
- Newspaper article from: Blood Weekly December 2, 2004 700+ words
...The LightCycler PCR assay detects and characterizes...evaluated real-time fluorescence PCR assays for the Roche...for the block cycler PCR assay," said researchers...Microbiology (Real-time fluorescence PCR assays for detection...
- Quantitative fluorescence-PCR recommended for rapid aneuploidy testing.
- Newspaper article from: Genomics & Genetics Weekly December 31, 2004 700+ words
...They've tested 7,720 prenatal samples over the past 3 years "for trisomies 13, 18, and 21 using a quantitative fluorescence-PCR (QF-PCR) approach." The team reported, "The abnormality rate was 5.7%. There were no misdiagnoses for nonmosaic...
- Evaluation of PCR assay for common endogenous plasmid and major outer membrane...
- Magazine article from: Indian Journal of Ophthalmology Satpathy, Gita Mohanty, Sujata Panda, S. April 1, 1999 700+ words
...trachomatis. Methods: In this study PCR assay was used to amplify the 517bp region...antigen detection. Results: The plasmid PCR assay was positive in 95 (53.37%) of the...marginally more sensitive, the plasmid PCR assay has the potential for wider use in the...
- Novel PCR assay useful for rapid detection of emetic Bacillus cereus.
- Newspaper article from: Genomics & Genetics Weekly October 29, 2004 700+ words
...com & NewsRx.net) -- A novel PCR assay is useful for the rapid detection of...According to recent research from Japan, "A PCR assay for the detection of Bacillus cereus...brain heart infusion medium. "This PCR assay enabled us to detect the bacteria of...
- Real-time PCR assay quantifies Plasmodium infection in mosquitoes.
- Newspaper article from: Obesity, Fitness & Wellness Week August 28, 2004 700+ words
...Scientists have developed a real-time PCR assay for quantifying Plasmodium falciparum...we describe a real-time quantitative PCR assay that is rapid, target-specific, and...University of Glasgow. "The real-time PCR assay utilizes the LightCycler platform and...
- THE IMMUNE RESPONSE CORPORATION PCR ASSAY CORRELATES VIRAL BURDEN WITH CLINICAL...
- Press release article from: PR Newswire March 5, 1992 700+ words
THE IMMUNE RESPONSE CORPORATION PCR ASSAY CORRELATES VIRAL BURDEN WITH CLINICAL...of HIV infection using a quantitative PCR assay developed by the company. Patients in...quantitative polymerase chain reaction (PCR) assay developed by Immune Response to evaluate...
- Real-Time PCR Assay for Monitoring Virus Load.
- Newspaper article from: Blood Weekly February 1, 1999 700+ words
...real-time polymerase chain reaction (PCR) assay proved useful for diagnosis of Epstein...Kimura and colleagues used a real-time PCR assay to quantify the amount of EBV DNA in...Barr virus load by using a real-time PCR assay." Journal of Clinical Microbiology...