Na,K-ATPase beta 3 subunit suppresses T and B lymphocyte activation.

2003 FEB 5 - (NewsRx.com & NewsRx.net) -- "In order to identify new molecules involved in regulation of T cell proliferation, we generated various monoclonal antibodies (mAb) by immunization of mice with the T cell line Molt4. We found one mAb (termed P-3E10) that down-regulated the in vitro T cell proliferation induced by CD3-specific OKT3 mAb. The P-3E10 mAb was also able to inhibit IFN-gamma, IL-2, IL-4 and IL-10 production of OKT3-activated T cells," researchers in Thailand and Austria report.

"The antigen recognized by P-3E10 mAb is broadly expressed on all hematopoietic as well as on all non-hematopoietic cell lines tested so far," reported Sawitree Chiampanichayakul and colleagues at Chiang Mai University and the University of Vienna. "Within peripheral blood leukocytes, the P-3E10 antigen was detected on lymphocytes, monocytes and granulocytes. Human umbilical vein endothelial cells (HUVEC) also scored positively. By evaluating the effect of P-3E10 mAb on these cell types we found that it also inhibited anti-IgM-induced B cell proliferation. However, it did not block growth factor-mediated proliferation of HUVEC, and spontaneous proliferation of SupT-1, Jurkat, Molt4 and U937 cell lines. Moreover, it did not influence phagocytosis of ...