Cost-precision ratios of strategies for antibody level measurement evaluated.

2003 FEB 5 - (NewsRx.com & NewsRx.net) -- According to recent research from Sweden, "Standardization and design of optimally reproducible strategies for the measurement of serum antibody levels by ELISA can present significant problems. In this article, we present a theoretical analysis of two common calculation methods in ELISA analysis (parallel line and reference line models), together with a new model, termed wPLL (a least squares-weighted modification of the parallel line model)."

"The subject of required precision in relation to the marginal costs of increased precision has not been well explored," commented Katarzyna Grabowska and colleagues at MAS University Hospital in Malmo. "We compared the three different calculation methods of expressing ELISA results based on the relationship between the dose response curves obtained from the reference serum and the test samples in two viral antibody determination systems (human papillomavirus and measles). The three methods were evaluated for inter- and intraassay precision using the coefficient of variation in ...