Tumour suppressor genes do not regulate the cell cycle. (Echo).(Brief Article)

A study of thc effects of hamartin and tuberin on cell growth suggests that tuber in acts as a tumour suppressor gene--but not by regulating the cell cycle. The researchers induced transient overexpression of hamartin and tuberin in cell cultures from a human kidney (HEK293) cell line by transfection. They used constructs of the TSC1 gene for hamartin and TSC2 gene for tuberin to transfect the cultures, separately and together, and constructs for three common TSC2 mutants They measured cell growth with two colour fluorescence activated cell sorting to analyse the percentage of cells in each growth phase.

TSC2, alone or with TSC1, produced a significant increase in G1 …