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Complete DNA sequences of Oka varicella vaccine and its parental virus compared.
2003 JAN 8 - (NewsRx.com & NewsRx.net) -- Osaka University microbiologists have compared the DNA sequences of the Oka varicella vaccine virus (V-Oka) and its parental virus (P-Oka).
They found "42 base substitutions, which led to 20 amino acid conversions and length differences in tandem repeat regions (R1, R3, and R4) and in an origin of DNA replication."
"Amino acid substitutions existed in open reading frames (ORFs) 6, 9A, 10, 21, 31, 39, 50, 52, 55, 59, 62, and 64. Of these, 15 base substitutions, leading to eight amino acid substitutions, were in the gene 62 region alone. Further DNA sequence analysis showed that these substitutions were specific for V-Oka and were not present in nine clinical isolates," said Y. Gomi and colleagues.
They reported that "[t]he immediate-early gene 62 product (IE62) of P-Oka had stronger transactivational activity than the mutant IE62 contained in V-Oka in 293 and CV-1 cells.
"An infectious center assay of a plaque-purified clone (S7-01) from the V-Oka with eight amino acid substitutions in ORF 62 showed smaller plaque formation and less-efficient virus-spreading activity than did P-Oka in human embryonic lung cells. Another clone (S-13) with only five substitutions in ORF 62 spread slightly faster than S7-01 but not as effectively as ...
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