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Risk for hepatitis C virus in semen presents need for laboratory caution.
2003 JAN 2 - (NewsRx.com & NewsRx.net) -- by Sonia Nichols, senior medical writer - Research collaborators in France say low levels of hepatitis C virus (HCV) can be detected in semen. They recommend that precautions precluding the risk of viral contamination should be used in laboratories that perform work for patients who undergo assisted reproduction.
The authors of a new study used a modified reverse-transcription polymerase chain reaction (RT-PCR) technique to detect HCV in semen. Although low levels of HCV RNA could be detected in a few semen aliquots, it could not be detected in concentrated semen samples. This led the authors to suggest that the risk for nosocomial transmission during assisted reproduction procedures with infected semen may be negligible but still possible.
"This study included 50 semen samples from 35 HCV-positive men, with active viral replication assessed by RT-PCR, collected the day of oocyte retrieval and used for assisted reproduction," said N.G. Cassuto of the Laboratoire Drouot in Paris, France.
The semen samples, plus samples that were purified down to 45% and 90% with centrifugation, were held in frozen storage before being tested for viral RNA with a new RT-PCR technique. The technique removed the effects of seminal amplification inhibitors, according to Cassuto and colleagues.
Despite finding HCV RNA in the semen samples of 5 of 35 HCV-positive men, investigators detected HCV RNA in only one 45% fraction, and in none of the 90% fractions. Embryo culture media ...
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