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Peptide extensions simply, effectively produce detoxified CT derivatives.(cholera toxin)
2002 DEC 4 - (NewsRx.com & NewsRx.net) -- Researchers in Mexico have described a simple procedure to detoxify cholera toxin that maintains immunoadjuvanticity.
"Peptides related to the heat-stable enterotoxin STa were fused to the N terminus of the A-subunit of cholera toxin (CTA) to explore whether peptide additions could help generate detoxified cholera toxin (CT) derivatives. Proteins carrying APRPGP (6-CTA), ASRCAELCC-NPACPAP (16-CTA), or ANSSNYCCELCCNPACTGCYPGP (23-CTA) were genetically constructed. Using a two-plasmid system these derivatives were coexpressed in Vibrio cholerae with cholera toxin B-subunit (CTB) to allow formation and secretion of holotoxin-like molecules (engineered CT, eCTs)," explained J. Sanchez and colleagues, University Autonoma Estado Morelos.
"Purified eCTs maintained all normal CT properties yet they were more than 10-fold (eCT-6), 100-fold (eCT-16), or 1000-fold (eCT-23) less enterotoxic than wild-type CT," the researchers reported.
"The inverse correlation between enterotoxicity and peptide length indicated sterical interference with the ADP-ribosylating active site in CTA. This interpretation agreed with greater than 1000-fold reductions in cAMP induction, with reductions, albeit not proportional, in in vitro agmatine ADP-ribosylation, and was supported by molecular simulations."
Additionally, the researchers said "[i]ntranasal immunization of mice demonstrated that eCTs retained their inherent immunogenicity and ability to potentiate immune responses to a ...
- Cholera toxin protects against Clostridium difficile toxin A.
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...NewsRx.com & NewsRx.net) -- Cholera toxin protects against action by Clostridium...intestinal fluid secretion induced by the cholera toxin (CT) and Clostridium difficile toxin...antisecretory factor induced via action of cholera toxin on the enteric nervous and immune system...
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...com & NewsRx.net) -- The cholera toxin translocates to induce toxicity. According...research from the United States, "Cholera toxin (CT) and related AB[subscript...Sciences (The intracellular voyage of cholera toxin: going retro. Trends Biochem Sci...
- Cholera toxin B subunit may not be helpful as adjuvant in pertussis vaccine.
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...NewsRx.com & NewsRx.net) -- Cholera toxin B subunit may not be helpful as an...microgram) with and without recombinant cholera toxin B subunit (rCTB; 10 microgram) as...co-administration with recombinant cholera toxin B subunit as an adjuvant. Vaccine...
- Cholera toxin fights autoimmune disease.
- Magazine article from: Science News Raloff, Janet April 22, 1995 700+ words
...begun harnessing a fragment of the cholera toxin in a novel approach to thwarting autoimmune...recognized as foreign by attaching it to a cholera toxin fragment. When fed to animals, a...Of the two primary components of cholera toxin, only the A chain is actually poisonous...
- Effects of cholera toxin on immunity and its application demonstrated.
- Newspaper article from: Drug Week July 16, 2004 700+ words
...amp; NewsRx.net) -- Effects of cholera toxin on innate and adaptive immunity and...to recent research from Ireland, "Cholera toxin (CT) is a potent vaccine adjuvant...Journal of Leukocyte Biology (Effects of cholera toxin on innate and adaptive immunity and...
- Modification of cholera toxin B subunit enhances expression in plants.
- Newspaper article from: Drug Week May 7, 2004 700+ words
...net) -- Modification of the cholera toxin B subunit coding sequence enhanced...expression in plants. "The cholera toxin B subunit (CTB) contains five...Breeding (Modification of the cholera toxin B subunit coding sequence to...
- Group B streptococcal protein rib and cholera toxin protect against infection.
- Newspaper article from: Health & Medicine Week April 19, 2004 700+ words
...group B streptococcal protein rib and cholera toxin B subunit confers protection against...administered with the recombinant cholera toxin B subunit, induces systemic immunoglobulin...group B streptococcal protein rib and cholera toxin B subunit confers protection against...
- Intracellular potentiation between two second messenger systems may contribute...
- Magazine article from: Gut Banks, M.R. Golder, M. Farthing, M.J.G. Burleigh, D.E. January 1, 2004 700+ words
...Gut 2004;53:50-57 Background: Cholera toxin (CT) acts on intestinal epithelial...contribute to the mechanism of action of cholera toxin induced secretory diarrhoea...diarrhoea primarily through the action of cholera toxin (CT) on the intestinal mucosa. On...
- Cholera toxin induces migration of dendritic cells.(Brief Article)
- Newspaper article from: Vaccine Weekly March 5, 2003 700+ words
...DCs). We tested whether uptake of the mucosal adjuvant cholera toxin (CT) or live Salmonella bacteria can induce DC migration...collaborators published their study in Infection and Immunity (Cholera toxin induces migration of dendritic cells from the subepithelial...
- Cholera toxin induces migration of dendritic cells.
- Newspaper article from: Gastroenterology Week March 3, 2003 700+ words
...DCs). We tested whether uptake of the mucosal adjuvant cholera toxin (CT) or live Salmonella bacteria can induce DC migration...collaborators published their study in Infection and Immunity (Cholera toxin induces migration of dendritic cells from the subepithelial...
Source: HighBeam Research, Peptide extensions simply, effectively produce detoxified CT...