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New method allows mass production of dendritic cells.
2002 DEC 4 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - Large-scale production of dendritic cells is possible through the use of a cell selector followed by culture in a sterile-bag system, according to researchers in California.
"Conventional methods for generating monocyte-derived dendritic cells for clinical trials utilize the property of plastic adherence to select monocytes from leukapheresis samples," said Vinod Pullarkat and colleagues at the University of Southern California in Los Angeles and Nexell Therapeutics in Irvine, California. "This method is labor-intensive and has the potential for contamination at various steps."
The investigators overcame the problems inherent in the plastic adherence method of generating large quantities of dendritic cells (DCs) by using a magnetic cell selection system for monocyte enrichment in sterile bags. Lipopolysaccharide was used for maturation of the dendritic cells after which the DCs were pulsed with a melanoma antigen gp100 peptide (Large-scale monocyte enrichment coupled with a closed culture system for the generation of human dendritic cells. Journal of Immunological Methods, 2002;267(2):173-183).
The yield of DCs generated by plastic adherence in tissue culture bags was 5% compared with 52% for DCs produced in sterile bags after magnetic selection. Yet, both methods produced DCs that exhibited potent expression of CD86, CD80, CD40, CD83, CD44, CD11c and CD58 and possessed similar ...
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