AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
RNA interference research valuable for studying malaria parasite gene expression.
2002 NOV 27 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - Results from RNA interference studies indicated that blocking the expression of two cysteine protease genes in the malaria parasite Plasmodium falciparum causes abnormalities in and inhibits growth of the organism.
"Malaria remains a public health problem of enormous magnitude, affecting over 500 million people every year," said Pawan Malhotra and colleagues at the International Centre for Genetic Engineering and Biotechnology in New Delhi. "Lack of success in the past in the development of new drug/vaccines has mainly been attributed to poor understanding of the functions of different parasite proteins. Recently, RNA interference (RNAi) has emerged as a simple and incisive technique to study gene functions in a variety of organisms."
Introduction of double-stranded RNA (dsRNA) into an organism can silence related gene expression. Called RNA interference (RNAi), this phenomenon can be exploited to study the morphological effects caused by specific genes (Double-stranded RNA-mediated gene silencing of cysteine proteases (falcipain-1 and -2) of Plasmodium falciparum. Molecular Microbiology, 2002;45(5):1245-1254).
Malhotra and associates employed RNAi to study the effects of silencing the falcipain-1 and -2 cysteine protease genes of P. falciparum and found results similar to those induced by treatment with the cysteine protease inhibitor E-64: parasite growth was obstructed and the parasite developed severe morphological defects and accumulated ...
- Synthetic cysteine protease inhibitors have potent antimalarial activity.
- Newspaper article from: Anti-Infectives Week January 12, 2004 700+ words
...net) -- A series of synthetic cysteine protease inhibitors have demonstrated potent...antimalarial chemotherapy are the cysteine protease hemoglobinases falcipain-2 and...peptidyl aldehyde and alpha-ketoamide cysteine protease inhibitors against these proteases...
- Proteoglycan testican-1 inhibits lysosomal cysteine protease cathepsin L.
- Newspaper article from: Health & Medicine Week November 24, 2003 700+ words
...testican-1 inhibits the lysosomal cysteine protease cathepsin L. "Testican-1, a...competitive inhibitor of the lysosomal cysteine protease, cathepsin L, with a K[subscript...the structurally related lysosomal cysteine protease cathepsin B," researchers in the...
- A new endogenous inhibitor of cysteine protease is discovered.
- Newspaper article from: Obesity, Fitness & Wellness Week September 18, 2004 700+ words
...specific, endogenous inhibitor of cysteine protease from Staphylococcus epidermidis...Staphostatins, a novel family of cysteine protease inhibitors with a unique mechanism...inhibitory specificity, limited to cysteine protease from the same species," wrote G...
- Fibroblasts convert IL-8 to a mature form by means of a cysteine protease.
- Newspaper article from: Proteomics Weekly July 28, 2003 700+ words
...means of a temperature dependent cysteine protease. According to recent research published...pH 6, was completely inhibited by cysteine protease inhibitors, antipain dihydrochloride...converting enzyme appears to be a cysteine protease." Ohashi and colleagues published...
- Cysteine protease-enriched proteins protect goats against Haemonchus.
- Newspaper article from: Biotech Week December 29, 2004 700+ words
...NewsRx.com & NewsRx.net) -- Immunization with cysteine protease-enriched protein fractions protected goats against Haemonchus...goats against Haemonchus contortus after immunization with cysteine protease enriched protein fractions. Vet Res, 2004;35(5...
- Leishmania mexicana cysteine protease, CPB2.8, induces potent Th2 responses.
- Newspaper article from: TB & Outbreaks Week April 1, 2003 700+ words
...com & NewsRx.net) -- The Leishmania mexicana cysteine protease, CPB2.8, induced a potent Th2 response in mice...in the Journal of Immunology (The Leishmania mexicana cysteine protease, CPB2.8, induces potent Th2 responses. J Immunol...
- A highly active cysteine protease, ervatamin A, was isolated from a plant.
- Newspaper article from: Proteomics Weekly June 16, 2003 700+ words
...com & NewsRx.net) -- A new, highly active cysteine protease called ervatamin A was isolated from the Indian plant Ervatamia...Purification and biochemical characterization of a highly active cysteine protease ervatamin A from the latex of Ervatamia coronaria. J Protein...
- Bitten plants deploy gut-rotting enzyme. (Corn Defenses).(corn variety uses...
- Magazine article from: Science News Milius, S. September 14, 2002 700+ words
...reason why. Corn plants under attack quickly accumulate a cysteine protease--a protein-slicing enzyme--surrounding the location...defense chemicals typically show up in 8 hours, but the cysteine protease surges in about an hour and remains at high concentrations...
