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Reproducible primate malaria transmission model developed by Colombian researchers.

Vaccine Weekly

| November 20, 2002 | COPYRIGHT 2002 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

2002 NOV 20 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - A research team at the University of Valle in Cali, Colombia, has developed a reproducible nonhuman primate model of malaria transmission using Aotus lemurinus griseimembra monkeys, according to researchers in Cali, Colombia.

"Characterization of an Aotus-P. falciparum cyclical transmission model has become a top priority as a result of the significant progress toward the development of preerythrocytic malaria vaccines," explained J. C. Zapata and colleagues. "However, reproducible transmission of infective P. falciparum sporozoites by mosquito inoculation has been difficult to achieve even in splenectomized monkeys."

The investigators intravenously inoculated intact A. lemurinus griseimembra monkeys with two different strains of P. falciparum sporozoites, a monkey-adapted strain (Santa Lucia) and a wild strain (Falciparum-Cali-Colombia-4, FCC-4), which had been collected from the salivary glands of laboratory-raised Anopheles albimanus mosquitoes. Parasitemia was monitored by mosquito xenodiagnosis.

A 16-day average prepatent period was found for the Santa Lucia strain and a 24-day prepatent period for the FCC-4 strain. Both strains had an average peak parasite density of approximately 900 parasites/microliter.

"The prepatent period, the peak of parasitemia, and the duration of patency were independent of the size of the sporozoite inoculum and ...

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