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FISH is a valuable tool for differentiating among chlamydial infections.(Fluorescence In Situ Hybridization )
2002 OCT 17 - (NewsRx.com & NewsRx.net) -- Microbiologists in Germany report reliable sensitivity and specificity of fluorescence in situ hybridization for detecting and differentiating among Chlamydia species.
According to Technical University of Munich researchers S. Poppert and colleagues, "Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial communities and has recently also been shown to be a valuable tool for the rapid detection of various bacterial pathogens in clinical specimens."
Poppert and team developed a "hierarchic probe set" they tested "for the specific detection and differentiation of chlamydiae, particularly C. pneumoniae, C. trachomatis, C. psittaci, and the recently described chlamydia-like bacteria comprising the novel genera Neochlamydia and Parachlamydia."
"The specificity of the nine newly developed probes was successfully demonstrated by in situ hybridization of experimentally infected amoebae and HeLa 229 cells, including HeLa 229 cells coinfected with C. pneumoniae and C. trachomatis," the researchers ...
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Source: HighBeam Research, FISH is a valuable tool for differentiating among chlamydial...