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Antibody-expressing lactobacilli capable of binding to Streptococcus mutans.(Brief Article)
2002 SEP 11 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - Lactobacilli expressing a fragment of an antibody specific for Streptococcus mutans exhibited the ability to bind S. mutans in vitro and in vivo, according to researchers in Europe.
Carina Kruger, at the Karolinska Institute in Sweden, and colleagues from England and the Netherlands engineered a vector that encoded the single-chain Fv (scFv) portion of the antibody specific for the S. mutans antigen I/II (SAI/II) adhesion molecule. The vector was incorporated into Lactobacillus zeae, which then expressed the antibody fragment on its surface.
Enzyme-linked immunosorbent assay (ELISA) revealed that the antibody fragments could bind SAI/II. Contact with the scFv-expressing lactobacillus caused the agglutination of S. mutans that expressed SAI/II but not of the S. mutans SAI/II knockout strain.
Through the use of scanning electron microscopy (SEM), the investigators demonstrated that the scFv-expressing lactobacillus bound directly to SAI/II-coated beads,
In a rat model for dental caries, the scFv-expressing lactobacillus reduced the number of S. mutans organisms and lowered the incidence of caries.
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