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Nondenatured parvovirus B19 antigen advances analytical technology.(Brief Article)
2002 AUG 7 - (NewsRx.com & NewsRx.net) -- by Maria G. Essig, MS, ELS, senior medical writer - Use of a nondenatured form of the parvovirus B19 antigen NS1 by researchers in Denmark significantly improved the sensitivity of analytical tests for detection of NS1 in clinical samples.
Parvovirus B19 (B19) is the causative agent of fifth disease (erythemia infectiosum) and is associated with arthopathy. The major structural proteins encoded by B19 are the capsid proteins VP1 and VP2; the major nonstructural protein is NS1, which is necessary for DNA replication. A denatured form of NS1 has been used in enzyme-linked immunosorbent assays (ELISA) and Western blot (WB) analysis to detect the presence of B19 in clinical samples, however the lack of conformational integrity of NS1 decreases the sensitivity of the tests.
Erik D. Heelgaard, Panum Institute, and his colleagues in Copenhagen used baculovirus vectors and several viral isolates to create recombinant NS1 that retained its conformational structure.
The investigators used ELISA to test the performance of this recombinant B19 NS1 and VP2 on 252 sera samples. In sera from patients with past B19 infection (i.e., sera were VP2 IgG-positive), ELISA demonstrated a significantly increased sensitivity in detection of B19 NS1 IgG when campared with WB (78% vs. 33%, respectively; p=0.001).
These results contradict "perpetuated claims that B19 NS1 IgG is detected primarily in patients with arthralgia or chronic infection," stated Heelgard and his collaborators (Detection of parvovirus B19NS1-specific antibodies by ELISA and Western blotting employing recombinant NS1 ...
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Source: HighBeam Research, Nondenatured parvovirus B19 antigen advances analytical...