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Nef-Deleted Vaccine Strain Replicates In CD4 Cells.(Brief Article)
2002 JAN 16 - (NewsRx.com & NewsRx.net) -- by Michael Greer, senior medical writer - A nef-deleted simian HIV (SIV) strain uses the same cells for replication as wild-type SIV, according to researchers in the United States and Europe.
"Deletion of the nef gene from simian immunodeficiency virus (SIV) strain SIVmac239 yields a virus that undergoes attenuated growth in rhesus macaques and offers substantial protection against a subsequent challenge with some SIV wild-type viruses," explained Dr. Christiane Stahl-Hennig and colleagues at the German Primate Center in Gottingen and other institutions in the U.S. and Europe.
This vaccine candidate replicates in CD4 cells, just like its wild-type counterpart, Stahl-Henning and coauthors found.
The researchers administered high doses of nef-deleted SIV to the tonsils of rhesus macaques. Cells expressing viral RNA were almost all CD4 cells, they said, with dendritic cells and macrophages becoming infected only rarely.
Administration of this potential vaccine led to rapid proliferation of the virus, particularly in T-cell rich areas of the lymph nodes. After two weeks, however, Stahl-Henning and coworkers observed a marked rise in the lymph node populations of perforin-positive cytotoxic T cells and natural killer cells. This was followed by sharp drops in viral loads, study data showed, and postmortem examinations after 26 weeks revealed extremely low levels of viral RNA in lymphoid tissue.
Most cells infected with this nef-deleted SIV strain were not in ...
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