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Synthetic Peptides Cause Significant Lysis Of HPV Targets.
2001 SEP 13 - (NewsRx.com & NewsRx.net) -- by N.R. Saltmarsh, staff medical writer - Researchers in the United States have identified possible vaccine candidates for human papillomavirus (HPV), using an algorithm that may have applications in the development of other cancer immunotherapies.
"Over 90% of cervical carcinomas express human papillomavirus (HPV) E6 and E7 proteins, noted M.R. Castellanos and colleagues. "These unique antigens are ideal targets for the development of cytotoxic T-lymphocytes (CTL) for anti-tumor immunotherapy."
Castellanos and team created a computer-assisted algorithm to predict which peptides from HPV-18 E6 and E7 proteins might bind to HLA-A2 molecules, synthesized these peptides, and determined their binding activity. Cytotoxicity assays were performed to assess specific cell lysis after HLA-A2(+)-irradiated stimulator cells pulsed with HPV-18 peptides were incubated with HLA-A2(+) peripheral blood mononuclear cells, the researchers reported.
Of 295 possible sequences, the algorithm predicted 10 peptides that would have a high probability of binding to HLA-A2, and Castellanos and coworkers analyzed the four strongest candidates. Two of the peptides induced significant lysis in HPV-18 peptide-pulsed targets, they reported ("Synthetic peptides induce a cytotoxic response against human papillomavirus type-18," Gynecologic Oncology, 2001;82(1):77-83).
"The combination of our computer-assisted algorithm and binding assay permits ...
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- Synthetic Peptides Cause Significant Lysis Of HPV Targets.(Brief Article)
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...stimulate CTL that are HLA-A2(+). The researchers...peptide sequences from HPV type 16 E5 with the primary...anchoring amino acids to HLA-A2 molecules. They synthesized...high binding affinity to HLA-A2. Three of the nine...According to the authors, HPV E5 is a key protein that...
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