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Optimal DNA Vaccination Comprises Secreted HCMV Glycoprotein B.(Brief Article)
2001 SEP 12 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - Researchers in Hungary have outlined an ideal regimen for DNA vaccination against human cytomegalovirus (HCMV) in a report in Vaccine.
Optimization of humoral and cellular immune responses involves a secreted form of HCMV glycoprotein B (gB), reported V. Endresz and colleagues at the University of Szeged. They compared immune responses of mice injected with plasmids VR-gB and VR-gB(Delta)tm - expressing the full-length membrane-anchored or secreted forms of human cytomegalovirus (HCMV)-glycoprotein B (gB), respectively - and VR-pp65 expressing the HCMV-phosphoprotein 65 (pp65).
Their findings were as follows:
* Pretreatment of mice with the local anesthetic bupivacaine did not enhance antibody production
* Interferon (IFN-)(alpha) coexpressed with the immunizing plasmids moderately increased the antibody response
* Antibody responses were higher in mice inoculated at three sites in the quadriceps than in mice inoculated at one site with the same dose and in the same muscle
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