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DNA Plasmid Protects Mice From Lethal Challenge.(Brief Article)
2001 SEP 5 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - A DNA plasmid vaccine offers 70%-80% protection against highly mutational influenza virus strains in mice, researchers in Japan have found.
"DNA vaccination is characterized by its preferential induction of the cytotoxic T cell lymphocyte (CTL) response and is expected to be a useful means of protection against viral infection," explained K. Okuda and colleagues.
The researchers studied the ability of an expression plasmid (pME18S-M) containing M1 and M2 genes of influenza A/PR/8/34 to induce CTL activity in BALB/c mice after vaccination via the intramuscular or intranasal route. Their results were published in Vaccine.
pME18S-M stimulated CTL and influenza-specific antibody responses although antibodies did not neutralize the influenza virus. The vaccine did, however, offer 70%-80% protection in the mice after challenge with a lethal dose of the A/WSN/33 and A/PR/8/34 influenza strains, which was 100% fatal in unimmunized mice, reported Okuda and coworkers ("Protective immunity against influenza A virus induced by immunization with DNA plasmid containing influenza M gene," Vaccine, 2001;19(27):3681-3691). ...
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...break into smaller pieces or recombine into different shapes. This work involves irradiating extra-cellular, non-living DNA plasmid in solution and assessing the damage. After irradiating the vials at various high doses, the resulting DNA fragments were...
- DNA Plasmid Production Scaled-Up.
- Newspaper article from: Applied Genetics News May 1, 1999 700+ words
Completion of a 3000 liter plasmid manufacturing run at a DSM Biologics (www.dsmbiologics.com) plant in Montreal, Quebec validated technology transferred from Megabios Corp. (863A Mitten Rd., Burlingame, CA 94010; Tel: 650/697-1900, Fax: 650/652-1990) and triggered a $250,000 milestone payment to
- Megabios Corp. and DSM Biologics Successfully Scale-Up DNA Plasmid Production.
- Press release article from: PR Newswire April 21, 1999 700+ words
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- Ultrastructural and immunological characterization of hepatitis C core...
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Abstract: Recently, it has been shown that a truncated HCV core (HCcAg) variant, covering the first 120 aa (HCcAg.120), interacts with plasmid DNA vaccine (pIDKE2), encoding the HCV structural proteins (HCcAg, E1 and E2). In the present work, HCcAg.120-pIDKE2 complexes, forming heterogeneous
- The Determination of the Orientation of a Glycostlase cDNA in a Recombinant DNA...
- Magazine article from: Bulletin of the South Carolina Academy of Science Nodelman, Amanda January 1, 2001 700+ words
The purpose of this research was to determine the orientation of a DNA insert sequence when placed in a recombinant backbone plasmid. Mini-preparations were performed to extract the DNA from the plasmids. Restriction enzyme XMA I was used to remove DNA from one plasmid; ligate was used to replace
- Nanoparticles enhance immunogenicity of tuberculosis DNA vaccine.
- Newspaper article from: Vaccine Weekly July 14, 2004 700+ words
...effects of pulmonary delivery of a new DNA plasmid encoding eight HLA-A*0201-restricted...Pulmonary administration of the DNA plasmid incorporated in chitosan nanoparticles...Medicine. This article was prepared by Vaccine Weekly editors from staff and other reports...
- Anti-cancer DNA vaccination is less effective in older mice than younger.(Brief...
- Newspaper article from: Vaccine Weekly April 16, 2003 700+ words
...vaccination with a HER-2/neu DNA plasmid to induce protective immunity...three times with a pCMVneuNT DNA plasmid, then presented challenge with...This article was prepared by Vaccine Weekly editors from staff and other...
- Juvaris collaborates with RNA-based gene-inhibition technology company.
- Newspaper article from: Vaccine Weekly June 9, 2004 700+ words
...lipids are formulated with noncoding DNA (plasmid) to create JuvImmune, a single...This article was prepared by Vaccine Weekly editors from staff and other reports. Copyright 2004, Vaccine Weekly via NewsRx.com & NewsRx...
- Nasal Flt3 ligand cDNA elicits dendritic cells for enhanced mucosal immunity.
- Newspaper article from: Vaccine Weekly May 5, 2004 700+ words
...compared with mice given nasal OVA plus DNA plasmid without the FL gene," investigators...Proteomics. This article was prepared by Vaccine Weekly editors from staff and other reports. Copyright 2004, Vaccine Weekly via NewsRx.com & NewsRx...
Source: HighBeam Research, DNA Plasmid Protects Mice From Lethal Challenge.(Brief Article)