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Second-Generation Vaccine More Immunogenic In Mouse Model.(Brief Article)
2001 AUG 22 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - A purified, inactivated vaccine (PIV) against Japanese encephalitis (JE) virus is as protective as the currently licensed vaccine in mice and is more immunogenic, say researchers working at the Walter Reed Army Institute of Research, Silver Spring, Maryland.
The JE-PIV was made from an attenuated strain of JE virus propagated in certified Vero cells, purified, and inactivated with formalin, said A.K. Srivastava and associates writing in Vaccine. The manufacturing process, which followed current GMP guidelines for the production of biologicals, efficiently generated a high yield of virus, essentially free of contaminating host cell proteins and nucleic acids, they added.
When combined with alum adjuvant and administered in two subcutaneous injections to mice, JE-PIV stimulated high levels of virus-neutralizing antibodies in a dose-dependent fashion and protected mice from challenge with live, virulent JE virus, reported Srivastava and coauthors ("A purified inactivated Japanese encephalitis virus vaccine made in Vero cells," Vaccine, August 2001;19(31):4557-4565).
"Compared with the existing ...
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Source: HighBeam Research, Second-Generation Vaccine More Immunogenic In Mouse Model.(Brief...