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B. Pertussis Fimbriae Potential Carrier Proteins For Hib Vaccines.(Bordetella pertussis)
2001 AUG 15 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - Conjugation of a Haemophilus influenzae type b (Hib) polysaccharide (PRP) to a protein carrier greatly improves its immunogenicity, and researchers in England have done just that with fimbriae isolated from Bordetella pertussis.
By providing protection in infants and subsequent antibody maturation upon boosting, said A. Crowley-Luke and colleagues, Public Health Laboratory Service, Salisbury, these fimbriae could be important protective antigens against whooping cough. The researchers assessed B. pertussis fimbriae as novel protein carriers for the Hib vaccine in rabbits.
They purified fimbriae (Fim2 and Fim3) from B. pertussis and dissociated them in 6 M guanidine hydrochloride, pH 10.5, to produce proteins of defined size then conjugated them to PRP using both carbodiimide-mediated coupling and reductive amination ("Formulation and characterisation of Bordetella pertussis fimbriae as novel carrier proteins for Hib conjugate vaccines," Vaccine, 2001;19(25-26):3399-3407).
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Source: HighBeam Research, B. Pertussis Fimbriae Potential Carrier Proteins For Hib...