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GFP-A2/CTB Complexes Could Broaden Horizons Of Cholera Toxin-Based Vaccines.(Brief Article)
2001 JUL 25 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - Researchers in the United States have come up with a novel method of assembling cholera toxin-like complexes in vitro, which could ultimately expand the potential of cholera toxin-based vaccines.
S.O. Hatic and colleagues, described the usefulness of cholera toxin as a mucosal adjuvant when coadministered with antigens and the current work on generating CT chimeras with reduced toxicity and better efficiency, in their paper in the journal Analytical Biochemistry.
"To date, chimeric forms of CT [cholera toxin] have been produced in bacterial strains by co-expressing the CT B subunit and a chimeric form of the CT A subunit consisting of a target protein antigen fused with the A2 polypeptide of CT," explained Hatic and coworkers.
They generated a chimeric protein consisting of green fluorescent protein (GFP) fused with polypeptide A2 to investigate the feasibility of assembling cholera toxin holotoxin-like complexes in vitro. Such complexes could expand the variety of antigenic compounds that could be incorporated into cholera toxin-based vaccines, noted Hatic and colleagues.
The researches were successful in creating GFP-A2/CTB ...
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Source: HighBeam Research, GFP-A2/CTB Complexes Could Broaden Horizons Of Cholera Toxin-Based...