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New Assay Convenient For Large-Scale Screening Efforts.(Brief Article)
2001 JUN 13 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - A simplified test to detect antibodies to Japanese encephalitis virus could make large-scale screening and vaccine development more convenient.
The virus, which is transmitted primarily by Culex mosquitoes between birds and animals but only accidentally to man, affects an estimated 45,000 people annually in Asia and causes significant morbidity and mortality, S.H. Ting and associates reported in the Journal of Virological Methods.
"Since dengue is also prevalent usually in Japanese encephalitis-endemic areas, all Japanese encephalitis-positive sera must be confirmed by detecting Japanese encephalitis-specific neutralizing antibodies," explained Ting and coworkers.
The standard for that detection has been the plaque reduction neutralization test, but it took a week and used six- or 24-well plates to confirm Japanese encephalitis virus-specific antibodies and thus was not ideal for large-scale detection.
The new, simplified assay can also detect and quantify virus-specific antibodies but uses 96-well ...
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