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Endotoxin Removal Delivers Promising Vaccine Candidate For M. catarrhalis.(Brief Article)
2001 JUN 6 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - By reducing the level of endotoxin in the UspA2 protein of Moraxella catarrhalis, researchers have come up with a promising candidate for a vaccine against the organism, which causes upper respiratory tract infections especially in immunocompromised people.
"Before a vaccine can be administered parenterally, the level of endotoxin must be reduced as much as possible," explained M.J. Fiske and associates.
The trouble was that the endotoxin was tightly complexed to the UspA2 protein and refused to yield to Triton X-100. When the researchers tried the zwitterionic detergents Zwittergent 3-12 and Zwittergent 3-14, they were able to dissociate the endotoxin and then to separate it using either ion exchange or gel filtration chromatography.
This two-step process reduced the level of endotoxin 20,000-fold, according to measurement with the limulus amoebocyte lysate assay ("Method for reducing endotoxin in Moraxella catarrhalis UspA2 protein preparations," J Chromatogr B Biomed Sci Appl, 2001;753(2):269-278).
Endotoxin reduction appeared to have little effect ...
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Source: HighBeam Research, Endotoxin Removal Delivers Promising Vaccine Candidate For M....