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Plasmid DNA Vaccination Stops Disease Progression In Mice.(Brief Article)
2001 MAR 28 - (NewsRx.com & NewsRx.net) --
by N.R. Saltmarsh, staff medical writer - Vaccination with plasmid DNA encoding glutamic acid decarboxylase 65, immunoglobulin GFc (IgGFc), and interleukin 4 (IL-4) appears able to stop the progression of diabetes in mice.
"We have investigated the use of plasmid DNA (pDNA) vaccination to elicit Th2 effector cell function in an antigen-specific manner and in turn prevent insulin-dependent diabetes mellitus (IDDM) in nonobese diabetic (NOD) mice," reported R. Tisch and colleagues at the University of North Carolina.
The researchers combined pDNA with a fusion protein consisting of a fragment of glutamic acid decarboxylase 65 (GAD65) linked to IgGFc and IL-4.
NOD mice injected with the pDNA encoding GAD65-IgGFc and IL-4 showed arrested progression of diabetes, whether they were at early or late preclinical stages of the disease. Tisch and team noted that GAD65 was critical to this response since control mice injected with pDNA encoding hen egg lysozyme-IgGFc and IL-4 instead of GAD65-IgGFc/IL-4 continued to develop diabetes.
The pDNA-encoded IL-4 was, in turn, necessary for GAD-65 activation ("Antigen-specific mediated suppression of beta cell autoimmunity by plasmid DNA vaccination," Journal of Immunology, 2001;166(3):2122-2132).
NOD IL-4(null) mice immunized with ...
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