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Enzyme Immunoassay Offers New Tool For Identifying Estrogen Metabolites.(hydroxyestrone and 16 alpha-hydroxyestrone which may contribute to developing breast cancer)
2001 MAR 8 - (NewsRx.com) -- by Julie Crawshaw, medical staff writer -- The estrogen metabolites hydroxyestrone (2OHE-1) and 16 alpha-hydroxyestrone (16OHE-1) may contribute to developing breast cancer, so it is important to be able to detect these substances.
Researchers investigated the reliability of a new procedure for measuring 2OHE-1 that uses an enzyme immunoassay (EIA). EIA uncovered differences among the study's subject groups when measurements were taken in a single run.
"Although under certain conditions the assay would not distinguish 2OHE-1 from estriol, or possibly 2-methoxyestrone, steroids such as 17 beta-estradiol, estrone, and 16OHE-1 should not interfere with the test," according to F.W. Spierto and colleagues at the U.S. Centers for Disease Control and Prevention.
Their study examined how well the EIA kit worked for measuring 2OHE- 1 levels in serum obtained from healthy men and women. Spierto et al. worked with specimens obtained from 18 men and 20 women, and "found that the within-run coefficients of variation (CVs) were approximately 20%, and the among-run CVs, 30%. Because the SD for the procedure is high, the limit of detection (LOD) was also high ...
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