AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
2001 FEB 15 - (NewsRx.com) -- by Michelle Marble, staff medical writer -- Researchers in Spain successfully transfected the female reproductive tract of mice using DNA/lipsome complexes.
"Mouse female genital tract was transfected in vivo using the beta-galactosidase reporter gene," stated N. Relloso and colleagues from Madrid. "To transfect the female tract, DNA/liposome complexes were injected through the infundibul um of the oviducts of adult, immature, and pseudopregnant females. Females which were in different stages of the ovarian cycle were also employed."
Relloso et al. published the results of their study in the journal Molecular Human Reproduction ("In vivo transfection of the female reproductive tract epithelium." Mol Hum Reprod. 2000;6(12):1099-l105).
The researchers analyzed transfection efficacy using histochemical, immunological, and molecular procedures, including Southern blotting, polymerase chain reaction (PCR), and gene sequencing. They found that the lower ...
Source: HighBeam Research, In vivo Gene Transfer to Epithelium Possible.