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Auxotrophic Mutants Evaluated as Vaccine Candidates.(Brief Article)
2001 FEB 14 - (NewsRx.com & NewsRx.net) --
- by Michelle Marble, staff medical writer -- Researchers in England report that disruption of genes responsible for virulence of Mycobacterium tuberculosis may result in mutants useful as vaccine candidates.
"Auxotrophic mutants of M. tuberculosis have been proposed as new vaccine candidates," wrote Debbie A. Smith and colleagues from the London School of Hygiene and Tropical Medicine. "We have analyzed the virulence and vaccine potential of M. tuberculosis strains containing defined mutations in genes involved in methionine (metB), proline (proC), or tryptophan (trpD) amino acid biosynthesis."
Smith et al. published their study in the journal Infection and Immunity ("Characterization of auxotrophic mutants of Mycobacterium tuberculosis and their potential as vaccine candidates," Infect and Immun, February 2001;69(2):1142-1150).
"The metB mutant was a prototrophic strain, whereas the proC and trpD mutants were auxotrophic for proline and tryptophan, respectively," continued the researchers. "Following infection of murine bone-marrow-derived macrophages, H37Rv and the metB mutant strain survived intracellularly for over 10 days, whereas over 90% of proC and trpD mutants were killed during this time."
The researchers evaluated the mutated strains in SCID mice. They observed that both the H37Rv and the metB mutant were highly virulent. Mice infected with these strains had median survival times (MST) of 28.5 and 42 days, respectively.
The infection caused by the proC mutant was significantly attenuated. The MST was 130 days for these animals. According to the researchers, the ...
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Source: HighBeam Research, Auxotrophic Mutants Evaluated as Vaccine Candidates.(Brief Article)