AccessMyLibrary provides FREE access to over 30 million articles from top publications available through your library.
Create a link to this page
Copy and paste this link tag into your Web page or blog:
Antigen May be Possible Vaccine Candidate.(Brief Article)
2001 FEB 14 - (NewsRx.com & NewsRx.net) --
- by Michelle Marble, staff medical writer -- According to a study from England, a major secreted mycobacterial antigen (Ag) may have potential as a candidate vaccine against tuberculosis.
"The role of CD8(+) cytotoxic T lymphocytes (CTL) in protection against tuberculosis in human disease is unclear," stated S.M. Smith and colleagues, University of London. "In this study, we stimulated the peripheral blood mononuclear cells of bacillus Calmette-Guerin (BCG)-vaccinated individuals with live Mycobacterium bovis BCG bacilli to establish short-term cell lines and then purified the CD8(+) T cells."
Smith et al. published the results of their study in the Journal of Immunology ("Human CD8(+) CTL specific for the mycobacterial major secreted antigen 85A," J Immunol, 2000;165(12):7088-7095).
"A highly sensitive enzyme-linked immunospot (ELISPOT) assay for single cell interferon (IFN)-gamma release was used to screen CD8(+) T cells with overlapping peptides spanning the mycobacterial major secreted protein, Ag85A," continued the researchers. "Three peptides consistently induced a high frequency of IFN-gamma responsive CD8(+) T cells, and two human leukocyte antigen (HLA)-A*0201 binding motifs, P48-56 and P242-250, were revealed within the core sequences."
The researchers isolated CD8(+) T cells which responded to the 9-mer epitopes by using ELISPOT within fresh blood using free peptide or by binding of HLA-A*0201 ...
- Antigen May be Possible Vaccine Candidate.
- Newspaper article from: TB & Outbreaks Week February 13, 2001 700+ words
...According to a study from England, a major secreted mycobacterial antigen (Ag) may have...CTL specific for the mycobacterial major secreted antigen 85A," J Immunol, 2000...peptides spanning the mycobacterial major secreted protein, Ag85A," continued the researchers...
- Antigen May be Possible Vaccine Candidate.(Ag85A may be a potential vaccine...
- Newspaper article from: Immunotherapy Weekly February 14, 2001 700+ words
...According to a study from England, a major secreted mycobacterial antigen (Ag) may have...CTL specific for the mycobacterial major secreted antigen 85A," J Immunol, 2000...peptides spanning the mycobacterial major secreted protein, Ag85A," continued the researchers...
- Neisserial autotransporter modulates other autotransporter processing.
- Newspaper article from: Health & Medicine Week December 22, 2003 700+ words
...identified the autotransporters IgA protease and App, a homologue of the adhesin Hap of Haemophilus influenzae, as the major secreted proteins. Two forms of both of these proteins were found in the culture supernatant of the wild-type strain, whereas...
- Plasma phospholipid transfer protein fused with green fluorescent protein is...
- Magazine article from: Biochemistry and Cell Biology Siggins, Sarah Ehnholm, Christian Jauhiainen, Matti Olkkonen, Vesa M. April 1, 2006 700+ words
...observed by SDS-PAGE is 80 kDa, because of extensive glycosylation (Day et al. 1994; Huuskonen et al. 1998). The major secreted forms of PLTP carry complex glycans at 6 potential N-glycosylation sites, and the protein also has a number of potential...
- Research on molecular biology reported by N.S. Kim et al.
- Newspaper article from: Life Science Weekly October 7, 2008 700+ words
...corresponded to cysteine proteinase (CysP), which is encoded for by Rep1 and EP3A. This result shows that CysP is a major secreted protease in rice cell suspension cultures following induction via sugar starvation. Intron-containing self-complementary...
- New life sciences study findings recently were reported by G.D. Wiens and...
- Newspaper article from: Biotech Week December 24, 2008 700+ words
...after divergence of the species; these factors include capsular polysaccharides, heme sequestration molecules, and the major secreted cell surface antigen p57 (also known as major soluble antigen). Examination of the genome revealed a number of ORFs homologous...
- New life sciences study findings have been reported by researchers at...
- Newspaper article from: Biotech Week April 8, 2009 700+ words
...secreted RNases. Supernatants from mutants inactivated for srnA completely lacked RNase activity, indicating that SrnA is the major secreted RNase of L. pneumophila. Although srnA mutants grew normally in bacteriological media and human U937 cell macrophages...
- The proinsulin C-peptide--a multirole model.
- Magazine article from: Experimental Diabetes Research Steiner, Donald F. January 1, 2004 700+ words
...released together into the circulation during active secretion (exocytosis) (Steiner et al., 2000a). These are the two major secreted products of the [beta] cell, but the secretory granules also contain a variety of other peptides, including small amounts...
