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New Culture System Allows Long-Term Replication of Hepatitis C Virus RNA.(Brief Article)
2001 FEB 14 - (NewsRx.com & NewsRx.net) --
- Sonia Nichols, staff medical writer -- Hepatitis C virus (HCV), with its many genotypes, is complicated to culture in vitro, thus the difficulty with developing a vaccine for this infection in humans.
Researchers in Germany now report they have grown subgenomic HCV for more than a year in cells cultured in the laboratory.
The report was issued in the February 2001 Journal of Virology, where researchers at Johannes-Gutenberg University of Mainz described their work on two replicon-harboring cell lines.
Their work grew out of previous reports of the successful culture of subgenomic HCV RNA in Huh-7 cells. Huh-7 is a human hepatoma cell line.
"Taking advantage of this cell culture system that allows analyses of the interplay between HCV replication and the host cell, in this study we characterized two replicon-harboring cell lines that have been cultivated for more than one year," said Thomas Pietschmann et al.
Pietschmann's research team did not observe cytopathogenicity, which would have resulted in limited proliferation or morphological changes, they said ("Characterization of cell lines carrying self-replicating hepatitis C virus RNAs," J Virol, 2001;75(3):1252-1264).
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