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New Combined PCR/Fluorescent Probe Technique Enables Rapid Genotyping of Varicella-Zoster Virus.(Brief Article)
2001 JAN 10 - (NewsRx.com & NewsRx.net) -- Researchers with the U.S. Centers for Disease Control and Prevention (CDC) have developed a method they call fluorescent resonance energy transfer (FRET) for quick genotyping of varicella-zoster strains.
Their technique, they explained, combines rapid-cycle polymerase chain reaction (PCR) with "wild-type-specific fluorescent probe melting profiles for product genotyping."
V.N. Loparev and colleagues discussed the FRET technique in the December 2000 issue of the Journal of Clinical Microbiology ("Rapid genotyping of varicella-zoster virus vaccine and wild-type strains with fluorophore-labeled hybridization probes," J Clin Microbiol, December 2000;38(12):4315-9).
"A region including the polymorphic site in VZV [varicella-zoster virus] open reading frame (ORF) 62 was amplified in the presence of two fluorescence-labeled hybridization probes," the research team wrote. "During the annealing step of the thermal cycling, both probes bound to their complementary sequences in the amplicon, resulting in resonance energy transfer, thus providing real-time fluorescence monitoring of PCR."
They reported ...
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Source: HighBeam Research, New Combined PCR/Fluorescent Probe Technique Enables Rapid Genotyping...