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12:00 MECHANISTIC INSIGHT OF FRAGMENTATION CHARACTERISTICS OF MACROLIDE ANTIBIOTICS, Victor Ibeanusi and Yassin Jeilani, Spelman College, Environmental Science and Studies. Macrolide antibiotics are composed of a large ring (14 to 16 carbons) on which several sugars are attached, some of these are amino sugars with diethylamino group. Mass spectrometric characterization of macrolide antibiotics has been a challenge because of low number of characteristic fragments in their collision induced dissociation. Tandem mass spectrometric fragmentation of these antibiotics show two types of dissociations: a) cleavage of glycosidic linkages attaching the sugars to the macrolide ring ...