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Research conducted at Tokyo University has updated our knowledge about life sciences.(Report)

Women's Health Weekly

| May 07, 2009 | COPYRIGHT 2009 NewsRX. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)Copyright

According to recent research from Chiba, Japan, "In the spermatogenesis, many nucleosides and nucleobases are needed for the salvage nucleotide biosynthesis. One of the roles of Sertoli cells is to provide such nutrients to spermatogenic cells located within the blood-testis barrier (BTB)."

"We have already shown that nucleoside transporters are expressed and are functional in primary-cultured rat Sertoli cells and TM4 cells derived from mouse testis. Here, we examined the uptakes of purine ([H-3]guanine) and pyrimidine ([H-3]uracil) nucleobases using TM4 cells. Uptakes of both nucleobases were time- and concentration-dependent, and kinetic analysis indicated the involvement of high-affinity transport systems. Uptake of uracil was significantly reduced in the absence of Na+, although guanine uptake was mainly mediated by a sodium-independent transport system in TM4 cells. Guanine uptake was inhibited by other purine nucleobases, but not by pyrimidine nucleobases. Only pyrimidine nucleobases reduced uracil uptake. In addition, mycophenolic acid, an inosine monophosphate dehydrogenase ...

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