Reports from C.E. Pope and co-researchers add new data to research in female infertility.

According to a study from the United States, "Our goals were to: (1) determine if domestic cat sperm could be sorted to high purity by flow cytometry after overnight shipment of cooled samples; (2) evaluate the efficiency with which sorted sperm could be used to generate cat embryos in vitro; and (3) determine if live kittens of predetermined sex could be produced after transfer of embryos derived by IVF using sorted sperm. 141 Semen samples (n = 5) from one male were extended in electrolyte-free solution and shipped overnight at 4 degrees C to the sorting facility. 14 Samples were adjusted to 75 x 10(6) sperm/mL and stained with Hoechst 33342. After 1 h at 34.5 degrees C, samples were adjusted to 50 x 10(6) sperm/mL with 4% egg yolk TALP + 0.002% food dye and sorted by high-speed flow cytometry."

"Later resort analysis confirmed purities of 94% and 83% for X- or Y-chromosome bearing sperm, respectively. Sorted sperm were centrifuged, resuspended in TEST yolk buffer and shipped overnight to the IVF laboratory. After IVF of in vivo matured oocytes with X chromosome bearing sperm, cleavage frequency was 62% (54/87). After IVF of IVM oocytes with control, X- or Y-chromosome bearing sperm, the incidence of cleavage was 42% (48/115), 33% (40/120), and 35% (52/150), respectively, and blastocyst development was 53% (21/40), 50% (11/22), and 55% (23/42), respectively (P > 0.05). On Day 2, 45 embryos produced by IVF of in vivo ...