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Study results from Brigham and Women's Hospital provide new insights into DNA research.
According to a study from the United States, "High-resolution melting curve analysis is an accurate method for mutation detection in genomic DNA. Few studies have compared the performance of high-resolution DNA melting curve analysis (HRM) in genomic and whole-genome amplified (WGA) DNA."
"In 39 paired genomic and WGA samples, 23 amplicons from 9 genes were PCR amplified and analyzed by high-resolution melting curve analysis using the 96-well LightScanner (Idaho Technology). We used genotyping and bidirectional resequencing to verify melting curve results. Melting patterns were concordant between the genomic and WGA samples in 823 of 863 (95%) analyzed sample pairs. Of the discordant patterns, there was an overrepresentation of alternate melting curve patterns in the WGA samples, suggesting the presence of a mutation (false positives). Targeted resequencing in 135 genomic and 136 WGA samples revealed 43 single nucleotide polymorphisms (SNPs). All SNPs detected in genomic samples were also detected in WGA. Additional genotyping and sequencing allowed the classification of 628 genomic and 614 WGA amplicon samples. Heterozygous variants were identified by non-wild-type melting pattern in 98% of genomic and 97% of WGA samples (P = 0.11). Wild ...
- Market profile: melting curve.(Brief Article)
- Newspaper article from: Instrument Business Outlook June 30, 2002 700+ words
Melting curve analysis is the process of using temperature...fragment can be determined by finding the melting curve. First, a highlighter, such as...melting peak. Software attached to the melting curve analysis instrument forms the data...
- Melting curve-based analysis of adjacent SNPs: a single, fluorescent-dye...
- Magazine article from: Bioscience Technology Froehlich, Thomas Hoffmann, Michael December 1, 2006 700+ words
Introduction Melting curve analysis (MCA) is a robust and...protocol * PCR amplification and melting curve analysis 10 ng of purified anonymous...by grouping samples with similar melting curve shape either by auto-calling or...
- Roche Diagnostics Licenses Melting Curve Analysis Technique to Agilent.
- Press release article from: Business Wire July 29, 2008 700+ words
...licensing agreement for use of Roche's melting curve analysis technology. Under the terms...develop and sell products based on melting curve analysis. Financial terms of the agreement...disclosed. "The licensing of the melting curve analysis technique to Agilent is one...
- Roche Diagnostics Licenses Melting Curve Analysis Technique to Agilent...
- Newspaper article from: Gastroenterology Week August 18, 2008 700+ words
...licensing agreement for use of Roche's melting curve analysis technology. Under the terms...develop and sell products based on melting curve analysis. Financial terms of the agreement...disclosed. "The licensing of the melting curve analysis technique to Agilent is one...
- Publication No. WO/2009/081965 Published on July 2, Assigned to ARKRAY for...
- News wire article from: US Fed News Service, Including US State News July 9, 2009 700+ words
GENEVA, July 9 -- Kosuke Kubo, has developed a melting curve analyzing method. The patent has been assigned to...Property Organization, the invention aims to describe a "melting curve analyzing method enabling automatic analysis for detecting...
- Melting Curve Analysis System. (New Products).
- Magazine article from: Bioscience Technology March 1, 2003 700+ words
...usage of a number of different fluorescent systems. A software suite logs all data from the system and produces differential melting curve peaks that are then autoscored and designated. Thermo Hybaid, Franklin, MA. 888-449-2243; thermohybaid.com * Write...
- Rapid identification of Bordetella pertussis pertactin gene variants using...
- Magazine article from: Emerging Infectious Diseases Makinen, Johanna Viljanen, Matti K. Mertsola, Jussi Arvilommi, Heikki He, Qiushui November 1, 2001 700+ words
...amplification (ASA) assay. Strains representing prn1-5 were further identified by a real-time PCR combined with the melting curve analysis of FRET probes and gel electrophoresis. Results were compared to those obtained by sequencing (15). The speed...
- The Clinical Chemistry Analyzers Market: Technology Trends, Business Challenges...
- Press release article from: Business Wire October 11, 2007 700+ words
...laboratory equipment industry is now available to its catalogue. Clinical Chemistry Analyzers www.reportlinker.com/p060838/PRBW-Clinical-Chemistry.html Clinical chemistry analysis is one of the most important areas within clinical...
Source: HighBeam Research, Study results from Brigham and Women's Hospital provide new insights...