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According to a study from the United States, "Anti-Mullerian hormone (AMH), also known as Mullerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries. based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH."
"Equine GCT (n = 27) and normal equine ovaries (n = 10) were examined by IHC, In addition, sera front lour mares with GCT were characterized for AMH bioactivity, based upon suppression of Mullerian duct development in the fetal rat. Immunolabeling with alpha-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing, follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. ...
Source: HighBeam Research, Researchers at University of California, Medical Department publish...