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Analysis of protein binding in the myeloid-lymphoid leukemia gene translocation breakpoint cluster region.(COMMUNICATIONS--UNDERGRADUATE)(Clinical report)
The Myeloid-Lymphoid Leukemia (MLL) gene fuses to greater than 50 different loci as the result of reciprocal translocations associated with several subtypes of human acute leukemia. In every case, the fusion point in MLL is within the same 8.3 kilobase pair (kbp) region, called the MLL breakpoint cluster region (bcr). A number of studies indicate the MLL bcr is susceptible to DNA cleaving agents, such as topoisomerase II (topo II) and DNase I, but there have been no reports of direct binding of such proteins in this region. In fact, despite the conserved nature of the breakpoint region, no definitive translocation mechanism has been described. A major goal in understanding MLL rearrangement is the identification of proteins that recognize specific DNA sequence or chromatin structure in the MLL bcr. Therefore, the objective of this study is to determine the general protein binding characteristics of the MLL bcr. We have used an electrophoretic mobility shift assay (EMSA) to analyze protein binding within specific regions of the MLL bcr. Protein binding was noted in the extreme boundaries of this region, but not in an internal region previously described as both a topo II cleavage site and a DNase I hypersensitive site. The limited mobility observed with the protein-DNA complexes indicates large proteins or protein complexes ...
- Analysis of protein binding in the MLL translocation breakpoint...
- Magazine article from: Proceedings of the North Dakota Academy of Science Super, Heidi J. Anderson, Alysa L. Aldrich, Aileen April 1, 2007 700+ words
...begun analyzing protein binding in the MLL bcr using electrophoretic...apparent region of protein binding within the MLL bcr. The region marks...lines. Ultimately protein binding will be compared throughout the MLL bcr with the final...
- Analysis of chromatin structure in the myeloid-lymphoid leukemia gene...
- Magazine article from: Proceedings of the North Dakota Academy of Science Horrell, Jeremy C. Aldrich, Aileen M. Anderson, Alysa L. Super, Heidi J. April 1, 2009 700+ words
...used to determine protein binding characteristics in the MLL bcr. Proteins were...region of the MLL bcr showed several discontinuous...are important for protein binding. Our results suggest...the limits of the MLL bcr. Binding of proteins...
- Novel method devised for prediction of drug plasma protein binding.
- Newspaper article from: Pharma Business Week August 9, 2004 700+ words
...accurate prediction of drug plasma protein binding. According to recent research from...a new method to predict the plasma protein-binding percent of structurally diverse pharmaceutical...drugs with experimental human plasma protein binding percent data," noted K. Yamazaki...
- In-vitro relationship between protein-binding and free drug concentrations of a...
- Magazine article from: Journal of Health Population and Nutrition Alam, M.A. Awal, M.A. Subhan, N. Mostofa, M. February 1, 2009 700+ words
...on albumin (5). Since numbers of protein-binding sites are limited, competition will...higher toxicity (10-12). Plasma protein-binding properties are related to plasma clearance...display a high range of values in plasma protein-binding, percentage of drug eliminated by...
- Many novel cAMP response protein binding sites present in E. coli.
- Newspaper article from: Drug Week May 7, 2004 700+ words
...Evolutionary comparisons suggest many novel cAMP response protein binding sites are present in Escherichia coli. According to a study...Evolutionary comparisons suggest many novel cAMP response protein binding sites in Escherichia coli. Proc Nat Acad Sci USA, 2004...
- TERT requires reverse transcriptase activity and 14-3-3 protein binding ability.
- Newspaper article from: Cancer Weekly May 6, 2003 700+ words
...mitochondria. We conclude that both RT activity and 14-3-3 protein binding ability are required for the antiapoptotic function of TERT...requiring reverse transcriptase activity and 14-3-3 protein binding ability. FASEB J, 2003;17(2):U154-U173). Additional...
- Capillary electrophoresis applied to study of drug-plasma protein binding.
- Newspaper article from: Drug Week November 28, 2003 700+ words
...electrophoresis frontal analysis applied to the study of drug-plasma protein binding. According to recent research published in the journal...Principles and applications for the study of drug-plasma protein binding. Electrophoresis, 2003;24(17):2903-2913). For...
- Ludwig Institute obtains United States patent.(Isolated anti-prion-protein...
- Newspaper article from: BIOTECH Patent News February 1, 2002 700+ words
...New York, NY) has patented an isolated anti-prion-protein binding protein that has a molecular weight of about 55 kD to about...described is a peptide derived from this isolated anti-prion-protein binding protein. Diagnostic uses for each of these molecules are...
